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Hide columns dynamically

The previous answers are using legacy DataTables syntax. In v 1.10+, you can use column().visible():

var dt = $('#example').DataTable();
//hide the first column
dt.column(0).visible(false);

To hide multiple columns, columns().visible() can be used:

var dt = $('#example').DataTable();
//hide the second and third columns
dt.columns([1,2]).visible(false);

Here is a Fiddle Demo.

Hide columns when the table is initialized

To hide columns when the table is initialized, you can use the columns option:

$('#example').DataTable( {
    'columns' : [
        null,
        //hide the second column
        {'visible' : false },
        null,
        //hide the fourth column
        {'visible' : false }
    ]
});